LOCUS       40924_43129        4428 bp DNA     circular SYN 18-DEC-2018
DEFINITION  PCR template vector pTF103 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Fukuda T, Ohya Y, Ohta K.
  TITLE     Conditional genomic rearrangement by designed meiotic recombination 
            using VDE (PI-SceI) in yeast
  JOURNAL   Mol. Genet. Genomics 278 (4), 467-478 (2007)
  PUBMED    17587055
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Fukuda T, Ohta K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2007) Tomoyuki Fukuda, RIKEN; 2-1 Hirosawa, Wako, 
            Saitama 351-0198, Japan (E-mail:tfukuda@riken.jp, 
            Tel:81-48-467-9277(ex.5552), Fax:81-48-462-4691)
REFERENCE   3  (bases 1 to 4428)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4428)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Genet.
            Genomics"; date: "2007"; volume: "278"; issue: "4"; pages: "467-478"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAY-2007) Tomoyuki Fukuda, RIKEN"; volume: " 2-1 Hirosawa, Wako,
            Saitama 351-0198, Japan (E-mail:tfukuda@riken.jp, 
            Tel:81-48-467-9277(ex.5552), Fax"; pages: "81-48-462-4691"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..180
                     /note="sequence containing the mutated recognition sequence
                     of VDE"
     promoter        187..402
                     /label=URA3 promoter
     CDS             403..1203
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     misc_feature    1348..1527
                     /note="sequence containing the mutated recognition sequence
                     of VDE"
     promoter        complement(1557..1575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1582..1598)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1740..2195
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2221..2325
                     /label=AmpR promoter
     CDS             2326..3183
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3357..3945
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4233..4254
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4269..4299
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4307..4323
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4331..4347
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4368..4386
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tggtacgaca tgtattccag gtgcttttgg ttgtggtaag accgttatct ctcaatcttt
       61 gtccaagtac tccaattctg acgccattat ctattctggc tgcggagaaa gaggtaatga
      121 aatggcagaa gtcttgatgg aattcccaga gttatatact gaaatgagcg gtactaaaga
      181 aagcttttca attcatcatt ttttttttat tctttttttt gatttcggtt tccttgaaat
      241 ttttttgatt cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga
      301 ttggtatata tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc
      361 caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat
      421 aaggaacgtg ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac
      481 gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag
      541 ttagttgaag cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact
      601 gatttttcca tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt
      661 ttactcttcg aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct
      721 gcgggtgtat acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc
      781 ccaggtattg ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc
      841 cttttgatgt tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag
      901 ggtactgttg acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga
      961 gacatgggtg gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta
     1021 gatgacaagg gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca
     1081 ggatctgaca ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta
     1141 gagggtgaac gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa
     1201 aactaaaaaa ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca
     1261 atttaattat atcagttatt acccgggaat ctcggtcgta atgattttta taatgacgaa
     1321 aaaaaaaaaa ttggaaagaa aaagcttgtg cttttggttg tggtaagacc gttatctctc
     1381 aatctttgtc caagtactcc aattctgacg ccattatcta ttctggctgc ggagaaagag
     1441 gtaatgaaat ggcagaagtc ttgatggaat tcccagagtt atatactgaa atgagcggta
     1501 ctaaagaacc aattatgaag cgtactactc gagggggggc ccggtaccca attcgcccta
     1561 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     1621 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     1681 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     1741 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     1801 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     1861 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     1921 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     1981 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     2041 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     2101 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     2161 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     2221 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2281 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     2341 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2401 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2461 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2521 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     2581 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2641 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2701 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2761 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2821 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2881 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2941 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     3001 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     3061 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     3121 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     3181 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3241 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3301 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3421 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3481 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4021 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4081 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4141 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4201 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     4261 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     4321 caatttcaca caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac
     4381 taaagggaac aaaagctgga gctccaccgc ggtggcggcc gctctaga
//
